In vitro fertilization (IVF) - ICSI technology is the second generation IVF technology, also known as intracytoplasmic sperm injection technology, which is widely referred to as the second generation IVF. Based on IVF-ET (IVF-ET), using microscopic techniques and in vitro fertilization embryo transfer (IVF-ET) technology, a sperm with excellent morphology and vitality is artificially selected and injected into the egg cytoplasm. Then, it is injected into the egg using a microneedle to form an embryo.

Compared to the first generation of IVF, the second generation of IVF has transitioned from "free love" to "arranged marriage". Usually, a person's egg cell needs to be surrounded by 5-10000 semen to complete the fertilization process. Therefore, the weak or weak have lost the opportunity to become fathers due to their inability to provide a good fertilization environment. Second generation IVF has opened up new avenues for male infertility and difficulty in fertilization, which is a blessing for male infertility. The first generation of IVF uses a combination of one egg and multiple sperm, placing the egg in an environment of 5000 to 20000 sperm, while the second generation of IVF only requires a few or dozens of sperm to achieve excellent natural binding. It is recommended to combine the best sperm with the egg cell.

The second-generation IVF ICSI technology is mainly aimed at male infertility:

(1) Severe oligospermia.

(2) Full of energy.

(3) There are two types of azoospermia, one is congenital absence of the vas deferens, and the other is testicular spermatogenesis disorder. The former method is to see sperm in testicular biopsy tissue, and then perform ICSI with semen removed from the testicles, followed by ICSI examination. With the improvement and improvement of the culture medium, the pregnancy rate gradually increases, and ICSI has become the most effective method for treating male azoospermia.

(4) Dead sperm.

(5) The man tested positive for anti sperm antibodies.

(6) Abnormal sperm acrosome.

(7) A small amount of sperm in alkaline urine can lead to ejaculation, such as retrograde ejaculation and ICSI.

(8) If fertilization fails and the first generation IVF cannot naturally fertilize the egg, then ICSI is the only option for further treatment by artificially injecting sperm directly into the zona pellucida or egg cells, avoiding the zona pellucida barrier.

The fertilization rate of IVF is generally around 50%. The first generation of IVF allows sperm and eggs to freely combine, while the second generation is artificially selected. The advantage of choosing ICSI technology is that the success rate of fertilization can reach around 90%.