What is the specific process of buying sperm for in vitro testing? With the improvement of medical equipment and technology, the success rate of the third generation IVF is as high as 65%. The success rate varies for each hospital, but the overall success rate is high. So, what are the steps in the IVF process? This will introduce you to some relevant knowledge.

1、 Complete process of buying sperm and making IVF: 1. Go to the reproductive center clinic to determine whether IVF is necessary and establish a medical record.

2. Improve the inspection before the sperm supply tube. One week before IVF enters, three certificates (ID cards of both parties, marriage certificate, and birth certificate) should be prepared and handed over to the nurse in the registration room for examination.

Before entering the cycle, couples should arrange time to attend in vitro fertilization pre education classes at the center as soon as possible.

4. Generally, on the 20th day of menstruation, come to the center for pre transplantation. According to the patient's condition, a controlled ovarian hyperstimulation plan is adopted.

5. Controlled superovulation: Generally, GnRHa is used to reduce FSH and LH in the body, and then HMG or FSH ovulation promoting drugs are taken to stimulate follicle growth. Adjust the dosage of the medication based on the patient's response to the medication. The number of eggs obtained varies depending on the patient's age and drug dosage.

6. Follicle monitoring: In order to evaluate the effectiveness of ovarian stimulation and determine the time for egg retrieval, it is necessary to follow the doctor's advice to come to the seventh floor of the outpatient clinic for follow-up on time (usually before 9am), use vaginal ultrasound to monitor follicle size, and adjust medication dosage in conjunction with blood hormone testing. When the diameter of one or more follicles is greater than 1.8cm and the number of follicles above 1.4cm is equal to the E2 value, human chorionic gonadotropin (hCG) can be injected to promote follicle maturation. Take eggs 34-36 hours after injection of hCG.

7. Egg retrieval: The general method of egg retrieval is to guide the retrieval of eggs through vaginal ultrasound under injection of analgesics or intravenous anesthesia. The egg retrieval needle passes through the vaginal fornix and reaches the ovary to remove the egg. Then, immediately transfer the egg to a culture dish containing embryo culture medium under a microscope and culture it in a 37 ℃ incubator

8. In vitro fertilization: Thawing the semen in the sperm bank and combining with the egg to form a fertilized egg. If natural fertilization is not possible, forced fertilization must be achieved through microinjection (referring to intracytoplasmic sperm injection).

9. Embryo transfer: After being cultured in vitro for 48-72 hours, the fertilized egg can develop into an 8-16 cell stage embryo. At this point, the number of embryos to be transferred is determined based on the patient's age, pregnancy status, and embryo quality. Excess embryos can be frozen. Embryo transfer does not require anesthesia. Currently, most embryos are transferred 2-3 days after fertilization.

10. Hormone supplementation after embryo transfer: Currently, progesterone can be injected to support the corpus luteum, and vaginal medication such as Ancitan or Sheraton can also be used.

14. On the 14th day after embryo transfer, urine and blood HCG were used to determine pregnancy.

On the 28th day after pregnancy, ultrasound examination is performed to determine the number of fetuses and the location of embryo implantation.

2、 The process steps of in vitro fertilization (IVF) are the optimization of sperm egg fusion using scientific technology to eliminate potential risks such as genetics. Performing IVF surgery mainly includes the following process steps:

Ovulation promotion: Women's eggs are like rare treasures, they can only ovulate one ovary per month. Because not every egg can combine with sperm, nor can every fertilized egg form an embryo, in order to improve pregnancy rate, it is necessary to undergo ovulation induction treatment in advance for women to promote synchronous growth of multiple follicles and remove multiple high-quality eggs at once.

3、 There are various options for inducing ovulation, such as long, short, and microstimulation options. The most commonly used is a rectangular table. This plan only provides ovulation induction injections starting from the previous luteal cycle, mainly targeting young women with good ovarian reserve function, with the highest probability of removing multiple eggs; Short programs are suitable for women who are older, have fewer follicles, and have poorer quality. The ovulation induction needle can be administered on the second day of their menstrual cycle, with a short duration but a small number of eggs.

Egg retrieval and sperm retrieval: Fix a slender specialized egg retrieval needle on the ultrasound probe. Under ultrasound detection, the doctor uses an egg retrieval needle to enter the ovary from the vagina, puncture mature follicles, and extract follicular fluid. Follicular fluid flows into various test tubes along the small tubes of the egg retrieval needle, and the egg retrieval is completed.

When retrieving eggs, sperm is taken from males and is also performed under intravenous anesthesia. The patient will not feel any pain and usually can complete it in more than ten minutes.

In vitro fertilization: After the sperm and egg are taken out, the sperm should be specially cleaned first. Because the semen may contain syphilis, hepatitis B, AIDS virus and other viruses, it is necessary to separate the sperm and semen. Afterwards, sperm and eggs are placed in artificially controlled culture media, and can choose to naturally combine or artificially inject sperm into the egg cells.